Rickettsia monacensis拉丁名 VR-1928™統(tǒng)一編號
Type strain. Genome sequenced by depositor.
Product category Bacteria
Classification Rickettsiaceae, Rickettsia
Strain designation IrR/Munich
Type strain Yes
Genome sequenced strain Yes
Isolation source Tick (Ixodes ricinus), isolated on June 12, 1998.
Geographical isolation Germany; Munich
Applications Infectious disease research
Vector-borne disease research
Product format Frozen
Storage conditions Vapor phase of liquid nitrogen
Comments
Genome sequenced by depositor
Handling information
Host
Vero (ATCC CCL-81)
Effects
CPE; cell rounding; cell sloughing; cell degeneration
Complete medium
DMEM (ATCC® 30-2002™) + 5% FBS (ATCC® 30-2020™)
Temperature
34°C
Incubation
6-11 days at 34°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer. A Giemsa stain may also be used to determine end of incubation.
Recommendation for infection
Plate cells 18-24 hours prior to infection and infect when cultures are 65-75% confluent. Remove DMSO from bacteria vial by centrifugation (180 x g for 10 minutes at 4°C). Remove supernatant and add 2 mL of fresh medium to the pellet. Pulse vortex for 1 minute. Remove medium from host cell culture and inoculate with bacteria (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1 to 1). Adsorb by centrifugation for 30 minutes at 800 x g at 22°C. Add agent growth medium following adsorption.
Key abbreviations
°C, Degrees Celsius
DMSO, Dimethyl sulfoxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Quality control specifications
Volume
1.0 mL
History
Deposited as
Rickettsia monacensis
Depositors
T Kurtti, University of Minnesota
Chain of custody
ATCC <-- T Kurtti
Type of isolate
Arthropod
Year of origin
1998
Cross references
GenBank NZ_LN794217.1 Rickettsia monacensis, strain IrR/Munic, complete genome.
