Botrytis cinerea Persoon
46522™
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category
Fungi
Strain designation
LB 1
Type strain
No
Isolation source
strawberry
Applications
Agricultural research
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Preceptrol
No
Handling information
Medium
ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 325: Malt extract agar (Blakeslee's formula)
ATCC Medium 336: Potato dextrose agar (PDA)
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
Incubate the inoculum at the propagation conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
