Dictyostelium reciprocatum var. transitum Cavender et al. 拉丁名
MYA-4869 編號(hào)
Product category Fungi
Classification Protozoa, Dictyosteliomycota, Dictyosteliomycetes, Dictyosteliales, Dictyosteliaceae, Dictyostelium, Dictyostelium reciprocatum
Strain designation 別名 OH 601
Type strain 模式菌株 Yes
Isolation source Soil/leaf mold samples from semi-evergreen rain forest Geographical isolation Guatemala; Tikal
Product format 提供形式 Frozen
Storage conditions 保藏溫度 -80°C or colder
Preceptrol No
Genotype Not available
Morphology On non-nutrient agar after 15 days at 25°C, Sporocarps branched and unbranched. Spores smooth, ellipsoidal, 5.25-6 x 3-3.75 µm.
Comments Grown in two-member culture with Escherichia coli (ATCC 23437)
Medium 培養(yǎng)基 ATCC Medium 579: LY Agar for Filobasidium
ATCC Medium 919: Non-nutrient agar
ATCC Medium 2432: wMY (weak Malt Yeast Extract)
Temperature 培養(yǎng)溫度 20-25°C
Atmosphere 需氧情況 Aerobic
Handling procedure Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.
If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.
Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).
Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).
Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2 to 3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly.
The sign of viability is noticeable typically after 20-30 days of incubation.
However, the time necessary for significant growth will vary from strain to strain.
Handling notes Type strain of the species. Grown in two-member culture with Escherichia coli ATCC
23437.
To speed recovery of cells, grow fresh bacteria on agar medium 24 hours before inoculating D. transitum culture. In addition, exposure to low light may also benefit growth.
Sequenced data 18S ribosomal RNA gene 5’end, partial sequence: TTAAGCCATGCATGTCTAAGTATAAATTTTTATATGATGAAACTGCAGACGGCTCATTACAACAGTAATAAACTAATAGACTTTCGGGTTTCATTACCTTTTGGATAACCGCAGTAAATCGGGGCTAATACATACAATCGAGGGCTGACTGTTTACGGAATGTCCGCGATTATTAGCATTCAACCAATACCTCTCGGGGTTTTGTGGTGAAACCGAATAATATTGCAGATCGAAGCTTCGGCTTCGACAAGTCTATTGTGTTACTGCCCTATCAACTTTCGATGGTACGGTATTGGCCTACCATGGTTGTAACGGGTAACGGGGAATTAGGGTTCGATTCCGGAGAGGGCGCCTGAGAAATGGCGACCACTTCTACGGAAGGCAGCAGGCGCGCAAATTACTCAATCCCAATACGGGGAAGTAGTGACAAGAAATATTAATGCCTATCCTCTTAGGAAGGTAATTAAAATGGGTCTAAACTAAATCCATTTTCTAATACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCATATACTAAATTTGTTGCGGTTAAAAAGCTCGTAGTTGAAGTAAAAGCCTTATTGGGTTAATAGGTCTTAGCCGACTTCGTCGGTTTAAGGCTTCCAATATGTGTTTTTTAAATTAATTCATGGATTAGGTTACTTTTGTAATTCTAGTTCTTTTGGGTGTTTCACTGTGAGAAAATTGTGGTGCTTAAAGCGGGCGTTTAGCCTGATCTTTTGCAGCATGGTATGATAGAACATGAC 18S ribosomal RNA gene 3’ end, partial sequence: CCTTGTTGGAGTAAGTTGTAGGTCGCAAGGTCTATGACACTTCGCTGGGTCGGTAATGTGAGTTGATAAATACGAAAAATTAAAAAAACTTCTTAGAGGGACTACCTGTGGCAAACAGGGGGAAGTTCGAGGCAATAACAGGTCTGTGATGCCCTTAGATACCTTGGGCCGCACGCGCGCTACAATGTAGTACGCAAAAAGCAATCCTGGTCCGGGAGGACTGGGTAATCATAAATTTACTACGTAACTGGGATTGATCTTTGTAATTTTTGATCATCAACGAGGAATTCCTTGTAAGCGCAAATCATTACTTTGTGCTGAATATGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATCGAATGATACGGTAAAGCCAACAGASynonyms
Raperostelium reciprocatum var. transitum (Cavender et al.) Baldauf et al.
Depositors J.C. Landolt
Chain of custody ATCC <-- J.C. Landolt; Shepherd University <-- J.C. Cavender
Type of isolate Environmental
MYA-4869 編號(hào)
Product category Fungi
Classification Protozoa, Dictyosteliomycota, Dictyosteliomycetes, Dictyosteliales, Dictyosteliaceae, Dictyostelium, Dictyostelium reciprocatum
Strain designation 別名 OH 601
Type strain 模式菌株 Yes
Isolation source Soil/leaf mold samples from semi-evergreen rain forest Geographical isolation Guatemala; Tikal
Product format 提供形式 Frozen
Storage conditions 保藏溫度 -80°C or colder
Preceptrol No
Genotype Not available
Morphology On non-nutrient agar after 15 days at 25°C, Sporocarps branched and unbranched. Spores smooth, ellipsoidal, 5.25-6 x 3-3.75 µm.
Comments Grown in two-member culture with Escherichia coli (ATCC 23437)
Medium 培養(yǎng)基 ATCC Medium 579: LY Agar for Filobasidium
ATCC Medium 919: Non-nutrient agar
ATCC Medium 2432: wMY (weak Malt Yeast Extract)
Temperature 培養(yǎng)溫度 20-25°C
Atmosphere 需氧情況 Aerobic
Handling procedure Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.
If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.
Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).
Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).
Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2 to 3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly.
The sign of viability is noticeable typically after 20-30 days of incubation.
However, the time necessary for significant growth will vary from strain to strain.
Handling notes Type strain of the species. Grown in two-member culture with Escherichia coli ATCC
23437. To speed recovery of cells, grow fresh bacteria on agar medium 24 hours before inoculating D. transitum culture. In addition, exposure to low light may also benefit growth.
Sequenced data 18S ribosomal RNA gene 5’end, partial sequence: TTAAGCCATGCATGTCTAAGTATAAATTTTTATATGATGAAACTGCAGACGGCTCATTACAACAGTAATAAACTAATAGACTTTCGGGTTTCATTACCTTTTGGATAACCGCAGTAAATCGGGGCTAATACATACAATCGAGGGCTGACTGTTTACGGAATGTCCGCGATTATTAGCATTCAACCAATACCTCTCGGGGTTTTGTGGTGAAACCGAATAATATTGCAGATCGAAGCTTCGGCTTCGACAAGTCTATTGTGTTACTGCCCTATCAACTTTCGATGGTACGGTATTGGCCTACCATGGTTGTAACGGGTAACGGGGAATTAGGGTTCGATTCCGGAGAGGGCGCCTGAGAAATGGCGACCACTTCTACGGAAGGCAGCAGGCGCGCAAATTACTCAATCCCAATACGGGGAAGTAGTGACAAGAAATATTAATGCCTATCCTCTTAGGAAGGTAATTAAAATGGGTCTAAACTAAATCCATTTTCTAATACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCATATACTAAATTTGTTGCGGTTAAAAAGCTCGTAGTTGAAGTAAAAGCCTTATTGGGTTAATAGGTCTTAGCCGACTTCGTCGGTTTAAGGCTTCCAATATGTGTTTTTTAAATTAATTCATGGATTAGGTTACTTTTGTAATTCTAGTTCTTTTGGGTGTTTCACTGTGAGAAAATTGTGGTGCTTAAAGCGGGCGTTTAGCCTGATCTTTTGCAGCATGGTATGATAGAACATGAC 18S ribosomal RNA gene 3’ end, partial sequence: CCTTGTTGGAGTAAGTTGTAGGTCGCAAGGTCTATGACACTTCGCTGGGTCGGTAATGTGAGTTGATAAATACGAAAAATTAAAAAAACTTCTTAGAGGGACTACCTGTGGCAAACAGGGGGAAGTTCGAGGCAATAACAGGTCTGTGATGCCCTTAGATACCTTGGGCCGCACGCGCGCTACAATGTAGTACGCAAAAAGCAATCCTGGTCCGGGAGGACTGGGTAATCATAAATTTACTACGTAACTGGGATTGATCTTTGTAATTTTTGATCATCAACGAGGAATTCCTTGTAAGCGCAAATCATTACTTTGTGCTGAATATGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATCGAATGATACGGTAAAGCCAACAGASynonyms
Raperostelium reciprocatum var. transitum (Cavender et al.) Baldauf et al.
Depositors J.C. Landolt
Chain of custody ATCC <-- J.C. Landolt; Shepherd University <-- J.C. Cavender
Type of isolate Environmental
