Malassezia slooffiae J. Guillot, Midgley et E. Guého 拉丁名
38593™ 統(tǒng)一編號(hào)
An ampoule containing viable cells suspended in cryoprotectant.
Product category 產(chǎn)品類別 Fungi
Product type 產(chǎn)品類型 Yeast
Strain designation 別名 683
Type strain 模式菌種 No
Isolation source 隔離源 Scalp
Product format 規(guī)格 Freeze-dried
Storage conditions 保存條件 2°C to 8°C
Specific applications：Biomedical Research and Development Material
Preceptrol：No
Comments：Occurrence in children
Medium 培養(yǎng)基
ATCC Medium 1072: Pityrosporum medium
ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 2737: Modified Leeming & Notman Agar (MLNA)
Temperature 培養(yǎng)溫度 37°C
Atmosphere 需氧條件 Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
1 Open an ampoule according to enclosed instructions.
2 From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3 Aseptically transfer the suspension back into the test tube of sterile distilled water.
4 Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
5 Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
6 Incubate the inoculum at the propagation conditions recommended.
7 Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
