Rhodobaca bogoriensis Milford et al. 拉丁名
700920 編號(hào)
Product category Bacteria Product type Extremophile
Strain designation 別名 LBB1
Type strain 模式菌株 Yes
Isolation 分離源 source Lake Bogoria Geographical isolation Kenya
Product format 提供形式 Freeze-dried
Specific applications Biotechnology
Preceptrol No
Extremophile type Alkaliphile Phototroph
Medium 培養(yǎng)基 ATCC Medium 2193: RV5 medium
Temperature 培養(yǎng)溫度 35°C
Atmosphere 需氧情況 Anaerobic
Incubation Under light Handling procedure
1. Put 6 to 8 ml of Medium #2193 into a 13x100 mm screw cap test tube (small). Add cysteine (3.0% stock concentration, 2 ml/100 ml medium) and then fill the test tube to capacity with Medium #2193. Seal the test tube with a screw cap.
2. Let the tube sit at room temperature for 30 minutes before inoculating it with the rehydrated culture.
3. Open the freeze?dried vial according to enclosed instructions.
4. Aseptically take 0.5 ml of the pre-reduced medium and rehydrate the pellet.
5. Transfer the rehydrated pellet back into the screw cap test tube and close tightly. (The test tube should be filled to capacity). One or two drops can be streaked out onto #2193 agar plates. This culture grows aerobically in the dark.
6. Incubate the culture at 35oC under a tungsten lamp.
7. Once growth has been established (three to six days), the culture should be transferred to fresh broth. If a large inoculum is used (10?20%) the medium does not need to be pre-reduced.
8. When examined microscopically, the cells are motile rods that occur singly, in pairs and short chainsDeposited as Rhodobaca bogoriensis Milford et al. Depositors MT Madigan
Type of isolate Environmental
Year of origin 1998
Cross references GenBank AF248638 16S rRNA sequence
