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Avian reovirus
VR-3364?

Avian reovirus strain 2177 is a virus that was isolated from infected chickens. This product is an ATCC manufactured and accessioned progeny of ATCC VR-2449 cited in US Pat. No. 5,525,342.
Product category
Viruses
Product type
Animal virus
Classification
Reoviridae, Avian orthoreovirus
Strain designation
2177
Isolation source
Commercially-reared chickens afflicted with inflammation of the hock joints.
Applications
Infectious disease research
Product format
Frozen
Storage conditions
-70°C or colder
Handling information
Host
UMNSAH/DF-1 (ATCC CRL-3586)
Effects
cell rounding; cell clumping; cell detachment
Complete medium
DMEM (ATCC 30-2002) + 2% FBS (ATCC 30-2020)
Temperature
37°C
Recommendation for infection
Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
DMEM, Dulbecco's Modified Eagle's Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Quality control specifications
Volume
1.0 mL
History
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