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Avian reovirus type 3
VR-857?

Avian reovirus type 3 strain 59 is propagated in primary chicken embryo fibroblasts. This strain was isolated from the intestine of a chicken with cloacal pasting.
Product category
Viruses
Product type
Animal virus
Classification
Reoviridae, Reovirus
Strain designation
59
Isolation source
Intestine from chicken with cloacal pasting.
Product format
Frozen
Storage conditions
-70°C or colder
Characteristics
Serotype
type 3
Comments
Deposited as Avian reovirus type 3. Pathogenic for newborn mice.
Mycoplasma contamination
Not detected
Handling information
Host
Primary Chicken Embryo Fibroblast
Effects
CPE; syncytia
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
2 to 7 days in a humidified 5% CO2 atmosphere
Recommendation for infection
Plate cells 24-48 hours prior to infection. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum.  End adsorption by adding virus growth medium.
Handling notes
 Deposited as Avian reovirus type 3. Pathogenic for newborn mice.
Key abbreviations
°C, Degrees Celsius
CEF, Chicken embryo fibroblast cells
CO2, Carbon dioxide
CPE, Cytopathic effect
MOI, Multiplicity of infection
History
Deposited as
Avian reovirus type 3
Depositors
BL Patel, University of Minnesota, College of Veterinary Medicine
Type of isolate
Animal
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