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Mycoplasma hominis (Freundt) Edward
43518™

Product category
Bacteria
Strain designation
W2
Type strain
No
Isolation source
Wound
Product format
Freeze-dried
Detailed product information
EXPAND ALL COLLAPSE ALL
General
Preceptrol
No
Characteristics
Serotype
1
Handling information
Medium
ATCC Medium 243: Mycoplasma medium
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
PROPAGATION PROCEDURE:

1. Follow instructions as suggested for the culturing of

          Mollicutes:

PROCEDURES FOR PROPAGATING MOLLICUTES:

a) Open the vial according to the enclosed instructions.

b) Using a Pasteur or 1.0 ml pipette, withdraw approximately 0.5 to 1.0 ml from a tube containing 5.0 ml.  Rehydrate the pellet.

c) Aseptically transfer this aliquot back into the tube.  Mix well.

d) Make serial dilutions by transferring 0.5 ml from the original tube to a tube containing 4.5 ml.  Repeat process by transferring 0.5 ml from the second to a third tube, etc.  Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth.  Many strains will die out rapidly once acid or alkaline conditions are reached.  It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze?drying process often inhibits growth.

e) Use an uninoculated tube of broth to serve as a control.

f)  Plates may be inoculated to check colonial morphology.  You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony-forming units.  However, not all strains do well on solid medium.

g) Incubate all tubes and plates under the recommended conditions and appropriate temperature.  The time necessary for growth will vary from strain to strain.  Growth on plates generally requires additional incubation.

h) Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both.

2. This strain starts to show turbidity in the first few dilution tubes after 24 to 48 hours.  Additional incubation is required for growth on solid agar.

1. Subsequent, fresh transfers grow more rapidly than the original culture.  This strain produces moderate turbidity.

Handling notes
Broth tubes may be incubated aerobically, but plates should be under 5% CO2.
          Colonies on #243 agar display the typical ?fried-egg? morphology.

History
Deposited as
Mycoplasma hominis (Freundt) Edward
Depositors
JS Lin