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Mycoplasma hominis (Freundt) Edward
43519™

Product category
Bacteria
Strain designation
10
Type strain
No
Isolation source
Vaginal swab of pregnant woman
Product format
Freeze-dried
Detailed product information
EXPAND ALL COLLAPSE ALL
General
Preceptrol
No
Characteristics
Serotype
2
Handling information
Medium
ATCC Medium 243: Mycoplasma medium
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
1. Follow instructions as suggested for the culturing of
          Mollicutes:

PROCEDURES FOR PROPAGATING MOLLICUTES:

a) Open the vial according to the enclosed instructions.

b) Using a Pasteur or 1.0 ml pipette, withdraw approximately 0.5 to 1.0 ml from a tube containing 5.0 ml.  Rehydrate the pellet.

c) Aseptically transfer this aliquot back into the tube.  Mix well.

d) Make serial dilutions by transferring 0.5 ml from the original tube to a tube containing 4.5 ml.  Repeat process by transferring 0.5 ml from the second to a third tube, etc.  Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth.  Many strains will die out rapidly once acid or alkaline conditions are reached.  It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze?drying process often inhibits growth.

e) Use an uninoculated tube of broth to serve as a control.

f)  Plates may be inoculated to check colonial morphology.  You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony-forming units.  However, not all strains do well on solid medium.

g) Incubate all tubes and plates under the recommended conditions and appropriate temperature.  The time necessary for growth will vary from strain to strain.  Growth on plates generally requires additional incubation.

h) Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both.

2. Tubes and plates are incubated in the atmosphere of 5% CO2.  The incubation temperature is 37oC.

3. Medium to light turbidity will appear in the first few dilution tubes within 48 hours.  Additional incubation will be required for colonies to appear on solid medium.

Handling notes
Colonies on #243 agar are entire, smooth, and circular.
Store vials at freezer temperatures until ready to use.

Additional information on this culture is available on the ATCC web site at www.atcc.org.

History
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