Leptospira interrogans (Stimson) Wenyon
23476™
Reference strain for serotype Autumnalis.
Product category
Bacteria
Strain designation
Akiyami A
Type strain
No
Geographical isolation
Japan
Product format
Frozen
Storage conditions
-80°C or colder
General
Preceptrol
No
Characteristics
Serotype
Autumnalis
Comments
Reference strain for serotype autumnalis
Handling information
Medium
ATCC Medium 1470: Modified Leptospira medium
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
TRANSFER IMMEDIATELY upon thawing by aseptically withdrawing cell suspension from vial and inoculating a 10-12 mL tube of Medium #1470, inserting the pipette just below the surface of the semi-solid medium. Aliquots of 0.5 ml from this tube may be used to inoculate additional tubes if needed. An aerobic blood plate may also be streaked to test for purity.
Incubate test tubes with screw caps lightly tightened along with the blood plate at 30°C under aerobic conditions.
Initial growth may take up to 7 to 14 days. Observe growth as a thin, tight band forming just below the surface of the medium. The band thickens as incubation continues. Cellular morphology under phase contrast shows motile flexible helical rods in singles.
The aerobic blood plate should show no signs of growth.
Handling notes
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
History
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