Fusarium solani (Martius) Saccardo
204260 ™
An ampoule containing viable cells suspended in cryoprotectant.
Product categoryFungi
Strain designationFMC 324Type strainNoIsolation sourceEggs, Panstrongylus geniculatusGeographical isolationVenezuela; CaracusProduct formatFrozenStorage conditions-80°C or colder
PreceptrolNo
Characteristics
Commentsopportunistic pathogen of insect eggs
Handling information
MediumATCC Medium 336: Potato dextrose agar (PDA)
Temperature25°C
Handling procedureFrozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly for up to 4 weeks. The time necessary for significant growth will vary from strain to strain.
