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Genomic DNA from Mycobacterium gordonae strain TMC 1327
35760D-5?

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Genomic DNA isolated from Mycobacterium gordonae Strain TMC 1327. This bacterial strain is also available as ATCC? Catalog No. 35760?.
Product category
Bacteria
Product type
Nucleic acid
Derived from
Mycobacterium gordonae TMC 1327 [Puntal] (ATCC 35760)
Organism
Mycobacterium gordonae Bojalil et al.
Type strain
No
Applications
Bioinformatics
Next-generation sequencing
Mass
5 μg
Product format
Dried
Shipping information
Stored in 1X TE buffer
Characteristics
Comments
This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications.
*the PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.
Handling information
Handling procedure
Centrifuge tube prior to opening to prevent loss of pelleted material
Rehydrate contents of vial with molecular grade H2O.
Place vial at 37°C for 1 hour or at 2°C to 8°C overnight.
For more complete rehydration and to fully recover DNA, incubate the sample overnight at 4°C while rocking; then incubate for 1 hour at 65°C.  Resuspending the dried DNA in ≥250 μL may give better results.
Handling notes
Genomic DNA isolated from bacteria is appropriate for PCR* and other molecular biology applications.
*The polymerase chain reaction (PCR) process is covered by patents owned by Hoffmann-LaRoche Inc. Use of the PCR process requires a license.
Quality control specifications
Total amount
Total DNA by PicoGreen? measurement was found to be approximately 5 μg.
Integrity
Integrity of DNA was determined by electrophoresis on a 1% agarose gel stained with SYBR Safe?, and was found to be of high molecular weight.
Functional tests
Functional activity was confirmed by PCR amplification of the 16S ribosomal RNA gene.
Identity
Identity confirmed by sequencing of 16S ribosomal RNA gene (first ~500 base pairs).
Verification method
Whole-genome Sequencing
History
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