Escherichia coli (Migula) Castellani and Chalmers
49696 ™
Product category
Bacteria
Classification
Enterobacteriaceae, Escherichia
Strain designation
HF4714
Type strain
No
Isolation source
Derived from a cross of existing strains
Product format
Freeze-dried
General
Specific applications
Bacteriophage host
Preceptrol
No
Handling information
Medium
ATCC Medium 1065: LB Agar/Broth, Miller
ATCC Medium 1065: LB Agar/Broth, Miller
ATCC Medium 1787: KC medium
Temperature
37°C
Handling procedure
1. Open vial according to enclosed instructions.
2. Using a single tube of #1065 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1065 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C for 24 hours.
Handling notes
ATCC® Medium #1787 is recommended for phage propagation. For formulation, see product sheet for ATCC® 49696-B1™. This strain also will grow on Nutrient Broth, Trypticase Soy Broth, or other commonly used media.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
History
Legal disclaimers
