Amanita muscaria (Linnaeus : Fries) Persoon ex Hooker
ATCC 62932
An ampoule containing viable cells suspended in cryoprotectant.
Product category Fungi
Strain designation BM 714
Type strain No
Isolation source
Humus under Norway spruce
Geographical isolation
Germany; West Germany
Product format
Frozen
Storage conditions
-80°C or colder
Preceptrol
No
Characteristics
Comments
Mycorrhiza
Handling information
Medium
ATCC Medium 336: Potato dextrose agar (PDA)
Temperature
24°C
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly for up to 4 weeks. The time necessary for significant growth will vary from strain to strain.
Deposited as
Amanita muscaria (Linnaeus : Fries) Persoon ex Hooker
Depositors
I Kottke
Type of isolate
Environmental
ATCC 62932
An ampoule containing viable cells suspended in cryoprotectant.
Product category Fungi
Strain designation BM 714
Type strain No
Isolation source
Humus under Norway spruce
Geographical isolation
Germany; West Germany
Product format
Frozen
Storage conditions
-80°C or colder
Preceptrol
No
Characteristics
Comments
Mycorrhiza
Handling information
Medium
ATCC Medium 336: Potato dextrose agar (PDA)
Temperature
24°C
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly for up to 4 weeks. The time necessary for significant growth will vary from strain to strain.
Deposited as
Amanita muscaria (Linnaeus : Fries) Persoon ex Hooker
Depositors
I Kottke
Type of isolate
Environmental
