-
平臺(tái)編號(hào)：bio-136772
-
國(guó)際編號(hào)：hkd-htlr9ni
-
拉丁屬名： Human TLR9 Reporter HEK293 Cells (NF-κB and IL-8)
-
規(guī)格：3-7 x 10e6 cells
-
用途：invivnogen原裝
-
服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
Human TLR9 Reporter HEK293 Cells (NF-κB and IL-8)
Notification: This cell line has been renamed. It was formerly known as "HEK-Dual? hTLR9 (NF/IL8)". The cat. code (hkd-htlr9ni) remains unchanged.
This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.NF-κB–SEAP and IL-8–Lucia reporter HEK293 cells expressing human TLR9
Signaling pathways in HEK-Blue-Lucia? hTLR9 cells
INVIVOGEN ALSO OFFERS:
? HEK-Blue? TLR cells: TLR reporter cells
? TLR9 ligands: for TLR studiesInvivoGen offers a human embryonic kidney 293 (HEK293)-derived cell line, specifically designed to assess the distinct role of the human Toll-like receptor 9 (hTLR9):— HEK-Blue-Lucia? hTLR9 cells* These cells were generated from the HEK-Blue-Lucia? Null cell line harboring two inducible reporter genes. This feature allows the double readout of the NF-κB/AP-1 pathway, by monitoring the SEAP (secreted embryonic alkaline phosphatase) or Lucia luciferase activities. HEK-Blue-Lucia? hTLR9 cells also stably express the hTLR9 genes. Due to a triple knockout (KO) of TLR3, TLR5, and TNFR, this cell line allows for the independent study of TLR9. Stimulation of HEK-Blue-Lucia? hTLR9 cells with TLR9 agonists (e.g. oligonucleotides (ODNs)) triggers the activation of the artificial NF-κB-inducible promoter and the subsequent production of SEAP. It also promotes the expression of Lucia luciferase, which is knocked in (KI) downstream of the endogenous (more physiological) IL-8 promoter (see figures). Of note, their parental cell line HEK-Blue-Lucia? Null may slightly respond to TLR9-specific agonists due to the weak endogenous expression of TLR9 in HEK293 cells (data not shown).IL-8 (interleukin 8) is a chemokine produced in response to TLR agonists in an NF-κB/AP-1-dependent manner [1-2]. This feature enables the double readout study of the NF-κB/AP-1 pathway, by monitoring the activity of SEAP and Lucia luciferase using QUANTI-Blue? Solution (SEAP detection reagent) or QUANTI-Luc? 4 Lucia/Gaussia (luciferase detection reagent), respectively. Thus, you may choose the readout depending on your laboratory equipment utilizing a spectrophotometer for SEAP or a luminometer for Lucia luciferase detection.
Toll-Like Receptor 9 (TLR9) is one of the most studied pattern recognition receptors (PRRs) for nucleic acid sensing. It is an endosomal receptor that triggers NF-κB- and IRF-mediated pro-inflammatory responses [3].
This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.NF-κB–SEAP and IL-8–Lucia reporter HEK293 cells expressing human TLR9
Signaling pathways in HEK-Blue-Lucia? hTLR9 cells
INVIVOGEN ALSO OFFERS:? HEK-Blue? TLR cells: TLR reporter cells
? TLR9 ligands: for TLR studiesInvivoGen offers a human embryonic kidney 293 (HEK293)-derived cell line, specifically designed to assess the distinct role of the human Toll-like receptor 9 (hTLR9):— HEK-Blue-Lucia? hTLR9 cells* These cells were generated from the HEK-Blue-Lucia? Null cell line harboring two inducible reporter genes. This feature allows the double readout of the NF-κB/AP-1 pathway, by monitoring the SEAP (secreted embryonic alkaline phosphatase) or Lucia luciferase activities. HEK-Blue-Lucia? hTLR9 cells also stably express the hTLR9 genes. Due to a triple knockout (KO) of TLR3, TLR5, and TNFR, this cell line allows for the independent study of TLR9. Stimulation of HEK-Blue-Lucia? hTLR9 cells with TLR9 agonists (e.g. oligonucleotides (ODNs)) triggers the activation of the artificial NF-κB-inducible promoter and the subsequent production of SEAP. It also promotes the expression of Lucia luciferase, which is knocked in (KI) downstream of the endogenous (more physiological) IL-8 promoter (see figures). Of note, their parental cell line HEK-Blue-Lucia? Null may slightly respond to TLR9-specific agonists due to the weak endogenous expression of TLR9 in HEK293 cells (data not shown).IL-8 (interleukin 8) is a chemokine produced in response to TLR agonists in an NF-κB/AP-1-dependent manner [1-2]. This feature enables the double readout study of the NF-κB/AP-1 pathway, by monitoring the activity of SEAP and Lucia luciferase using QUANTI-Blue? Solution (SEAP detection reagent) or QUANTI-Luc? 4 Lucia/Gaussia (luciferase detection reagent), respectively. Thus, you may choose the readout depending on your laboratory equipment utilizing a spectrophotometer for SEAP or a luminometer for Lucia luciferase detection.
Toll-Like Receptor 9 (TLR9) is one of the most studied pattern recognition receptors (PRRs) for nucleic acid sensing. It is an endosomal receptor that triggers NF-κB- and IRF-mediated pro-inflammatory responses [3].
