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大腸埃希氏菌
    大腸埃希氏菌
  • 平臺(tái)編號(hào):bio-136852
  • 國際編號(hào):ATCC 49980
  • 拉丁屬名: Escherichia coli
  • 規(guī)格:Freeze-dried
  • 用途:ATCC原裝進(jìn)口
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購 說明書
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

Escherichia coli (Migula) Castellani and Chalmers 拉丁名
49980 編號(hào)
Product categoryBacteria
Classification Enterobacteriaceae, EscherichiaStrain designationNCIMB 11187 [WP2]Type strainNoIsolation sourceMutant derived from strain B/RProduct formatFreeze-dried

Specific applications
Testing mutagens

Preceptrol No

Genotypetrp-Comments

Mutation induced by freeze-drying
Mutation induced by heliotrine and monocrotaline
Mutation induced by the fungicide captan
Ultraviolet dose-mutation relationships

Medium 培養(yǎng)基 ATCC Medium 3: Nutrient agar or nutrient broth
Temperature 培養(yǎng)溫度 37°C
Handling procedure

1. Open vial according to enclosed instructions.                            

2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate pellet. 

3. Aseptically transfer this aliquot back into the broth tube.  Mix well.

4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate. 

5. Incubate all tubes and plate at 37°C for 24 hours.

Handling notes

On #3 plates, colonies are entire, glistening, circular, and smooth.  The requirement for tryptophan has been checked.

Deposited asEscherichia coli (Migula) Castellani and ChalmersDepositorsNCIMBChain of custodyATCC <-- NCIMB <-- M.H.L. Green <-- E.M. Witkin

 

 

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