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平臺編號：bio-136886
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國際編號：ATCC 29148D-5
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拉丁屬名： Genomic DNA from Bacteroides thetaiotamicron strain VPI 5482
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規(guī)格：Dried 5μg
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用途：ATCC原裝進(jìn)口
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Genomic DNA from Bacteroides thetaiotamicron strain VPI 5482
Genomic DNA from Bacteroides thetaiotamicron strain VPI 5482 拉丁名
29148D-5 編號
Product category Bacteria Product type Nucleic acid
Derived from Bacteroides thetaiotaomicron VPI 5482 [CIP 104206T, E50, NCTC 10582] (ATCC 29148)
Organism Bacteroides thetaiotaomicron (Distaso) Castellani and Chalmers
Type strain 模式菌株 Yes
Genome sequenced strain Yes
Applications Bioinformatics Next-generation sequencing
Mass 規(guī)格 5 μg
Product format 提供形式 Dried Shipping information Stored in 1X TE buffer
Comments This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction
(PCR)* process and other molecular biology applications.
*The PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.
Genome sequenced strainHandling procedure Centrifuge tube prior to opening to prevent loss of pelleted material Rehydrate contents of vial with molecular grade H2O.
Place vial at 37°C for 1 hour or at 2°C to 8°C overnight. For more complete rehydration and to fully recover DNA, incubate the sample overnight at 4°C while rocking; then incubate for 1 hour at 65°C.
Resuspending the dried DNA in ≥250 µL may give better results.
Handling notes Genomic DNA isolated from bacteria is appropriate for PCR* and other molecular biology applications.
*The polymerase chain reaction (PCR) process is covered by patents owned by Hoffmann-LaRoche Inc.
Use of the PCR process requires a license.
Depositor of Source Strain: EP CatoTotal amount Total DNA by PicoGreen® measurement was found to be approximately 5 µg.
Purity (A260/A280) 1.6 to 2.0 Integrity Integrity of DNA was determined by electrophoresis on a 1% agarose gel stained with SYBR Safe™, and was found to be of high molecular weight.
Functional tests Functional activity was confirmed by PCR amplification of the 16S ribosomal RNA gene. Identity Identity confirmed by sequencing of 16S ribosomal RNA gene (first ~500 base pairs).
Verification method
Whole-genome SequencingDeposited as Bacteroides thetaiotaomicron (Distaso) Castellani and Chalmers Depositors ATCC
Cross references GenBank AE015928
Bacteroides thetaiotaomicron VPI-5482, complete genome.
