pHPV-16 purified plasmid DNA
45113D ™
pHPV-16 purified plasmid DNA is a clone of HPV16 that has been linearized at the unique BamHI site and cloned into the XhoI site of the pBluescript SK- vector. This product has applications for molecular biology.
Comments
Cloned from human papilloma virus type 16 by linearizing the virus at the unique BamHI site.
Full-length.
The insert contains the following restriction sites (nt from the vector KpnI site [nt according to GenBank accession K02718]): PstI--654 [6791], 870 [7007], 2646 [879], 5463 [3696], 6526 [4759], 7009 [5242]; KpnI--2651 [884], 7148 [5381]; EcoRI - 685 [6818], 1320 [7453].
BamHI+BglI will excise the insert with 205 nucleotides of the vector.
Restriction digests of the clone give the following sizes (kb): BamHI/BglI--8.0, 1.5, 1.2; PstI--3.6, 2.9, 1.8, 1.1, 1.0, 0.5; KpnI--4.4, 3.7, 2.7; EcoRI--7.0, 3.6, 0.6.
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE or water
Storage conditions
2°C to 8°C
Handling information
Handling notes
Restriction digests of the clone give the following sizes (kb): BamHI/BglI--8.0, 1.5, 1.2; PstI--3.6, 2.9, 1.8, 1.1, 1.0, 0.5; KpnI--4.4, 3.8, 2.7; EcoRI--7.0, 3.6, 0.6.
- ATCC staff
BamHI+BglI will excise the insert with 205 nucleotides of the vector.
The insert contains the following restriction sites (nt from the vector KpnI site [nt according to GenBank accession K02718]): PstI--654 [6791], 870 [7007], 2646 [879], 5463 [3696], 6526 [4759], 7009 [5242]; KpnI--2651 [884], 7148 [5381]; EcoRI--685 [6818], 1320 [7453].
- personal communication
Quality control specifications
Mycoplasma contamination
Not detected
History
Depositors
JE Marich, Syngene, Inc
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
