phi6-3
59134 ™
OrganismHomo sapiens, human
Clone typeCloneApplicationsMolecular biology
Product formatFreeze-driedShipping informationbacteria-free lysate
Specific applicationsStandard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C.
The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe.
Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information.
CommentsRestriction digests of the clone give the following sizes (kb): BamHI--25.0, 12.5, 2.0; SalI--19.5, 14.5, 10.0.
phi6-3 contains repeated sequences and must be prehybridized with total human DNA before it will detect single copy bands. Standard hybridization and washing conditions can be used, with a final wash in 0.1X SSC/0.1% SDS at 68C.
MspI detects 2 diallelic RFLPs. The constant band at 2.5 obscures the B2 allele when the entire phage is used as probe. Because the 2 loci are in total disequilibrium, use of locus A alone provides all available information.
Mycoplasma contaminationNot detected
Construct size (kb)44.0
Insert size (kb)14.5Type of DNAgenomicGene productDNA Segment, single copy [D11S85]AllelesA1, B2, A2, B1
DepositorsM LittCross referencesGenBank 165643
