24171 ™
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category
Fungi
Product type
Yeast
Strain designation
UCD 68-214 [CBS 140, DBVPG 6009, IFO 0698, IFO 0757, MUCL 30399, NRRL Y-2537]
Type strain
Yes
Isolation source
Air
Geographical isolation
Netherlands
Product format
Freeze-dried
General
Preceptrol
No
Handling information
Medium
ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 200: YM agar or YM broth
ATCC Medium 325: Malt extract agar (Blakeslee's formula)
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
Incubate the inoculum at the propagation conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
Quality control specifications
Sequenced data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
TGCGGAAGGATCATTAATGTATTTAGATTGAACCATAGGCGAAAGCCAGTGGTTCTTCTTTCATATCCATAACACCTGTGCACTGTTGGATGCTTGCATCCACTTTTAAACTAAACATTATTGTAACAAATGTAGTCTTATTATAACATAATAAAACTTTCAACAACGGATCTCTTGGCTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATGAAACCCTCAAACCCAAGTTTTGGATTTCGATCCATGCTTGAGTTTGGATTTGGATGTTTGCCGGTGATGAACCGACTCATCTTAAAAGTATTAGCTTGGATCTGTCTATATGACTGGTTTGACTTGGCATAATAAGTATTTTGCTGAGGACATCTTCGGATGGCCAGGACCTAGACTACTGTCTGCTAACTAAACCATCACTTTAAGTGCATCTTTGGATGTTACTCATTGTGTAACTTTGACATCTGGCCTCAAATCAAGTAGGACTACCCGCTGAACTAAAGCATATC
History
Deposited as
Cryptococcus magnus (Lodder et Kreger-van Rij) Baptist et Kurtzman
Synonyms
Cryptococcus laurentii var. magnus Lodder et Kreger-van Rij, Cryptococcus magnus (Lodder et Kreger-van Rij) Baptist et Kurtzman, Cryptococcus magnus var. magnus
Depositors
HJ Phaff
Type of isolate
Environmental
Cross references
GenBank EU252547 ITS including 5.8S rRNA gene
GenBank AF181851 26S ribosomal RNA gene, partial sequence
GenBank AF190008 internal transcribed spacer 1, 5.8S ribosomal RNA, and internal transcribed spacer 2, complete sequence
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