Candida albicans (Robin) Berkhout
18814
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category Fungi
Product type Yeast
Classification
Fungi, Ascomycota, Saccharomycotina, Saccharomycetes, Saccharomycetidae, Saccharomycetales, Candida
Strain designation
CBS 1949 [CCRC 22063, IFO 0759, JCM 2374, MUCL 29903, NRRL Y-2221, UCD 51-35]
Type strain
Yes; type strain of Syringospora claussenii
Geographical isolation
Denmark; Copenhagen
Product format Freeze-dried
Storage conditions 2°C to 8°C
Morphology
On YM medium at 25°C after 3 days, colonies white to dingy white dull, butyrous, smooth, margin entire. Cells hyaline, smooth, broadly ovoid, 7-9 X 4.5-6 µm. Pseudohyphae abundant
Medium
ATCC Medium 200: YM agar or YM broth
ATCC Medium 1245: YEPD
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
Incubate the inoculum at the propagation conditions recommended.
7Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
