Leptospira kirschneri Ramadass et al.
23469 ™
94/100
Bioz Stars
6 Product Citations
Product category
Bacteria
Strain designation
Moskva V
Type strain
No
Geographical isolation
USSR
Product format
Frozen
Storage conditions
-80°C or colder
General
Preceptrol
No
Characteristics
Serotype
grippotyphosa
Comments
Taxonomy
Allow 4-6 weeks for growth to appear.
Reference strain for serotype grippotyphosa
Handling information
Medium
ATCC Medium 1470: Modified Leptospira medium
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
Aseptically draw out cell suspension from vial and inoculate a 10x12 mL tube of Medium #1470, inserting the pipette just below the surface of the semi-solid medium. Aliquots of 0.5 mL from this tube may be used to inoculate additional tubes if needed. An aerobic blood plate may also be streaked to test for purity.
Incubate test tubes with screw caps lightly tightened along with the blood plate at 30°C under aerobic conditions.
Initial growth may take four to six weeks. Observe growth as a white, tight band forming just below the surface of the medium. The band thickens as incubation continues. Subsequent transfers grow in 10 to 14 days.
The aerobic blood plate should show no signs of growth.
Handling notes
ATCC Medium #1490 is a semi-solid medium.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
History
Legal disclaimers
