Leptospira borgpetersenii Yasuda et al.
23481 ™
92/100
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3 Product Citations
Product category
Bacteria
Strain designation
Perepelicin
Type strain
No
Isolation source
Bovine
Geographical isolation
USSR
Applications
Agricultural research
Product format
Frozen
Storage conditions
-80°C or colder
General
Preceptrol
No
Characteristics
Serotype
tarassovi
Comments
Taxonomy
Reference strain for serotype tarassovi
Handling information
Medium
ATCC Medium 1470: Modified Leptospira medium
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
TRANSFER IMMEDIATELY upon thawing by aseptically withdrawing cell suspension from vial and inoculating a 10-12 mL tube of Medium #1470, inserting the pipette just below the surface of the semi-solid medium. Aliquots of 0.5 mL fromthis tube may be used to inoculate additional tubes if needed. An aerobic blood plate may also be streaked to test for purity.
Incubate test tubes with screw caps lightly tightened along with the blood plate at 30°C under aerobic conditions.
Initial growth may take up to 4-6 weeks. Observe growth as a thin, tight band forming just below the surface of the medium after 2-4 weeks of incubation. The band thickens as incubation continues. Cellular morphology under phase contrast shows active, spiral cells with flexing motility.
The aerobic blood plate should show no signs of growth.
Handling notes
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
History
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