Meyerozyma guilliermondii (Wickerham) Kurtzman et M. Suzuki
90877 ™
An ampoule containing viable cells (e.g. yeast cells, spores) suspended in cryoprotectant.
Product category
Fungi
Product type
Yeast
Classification
Fungi, Dikarya, Ascomycota, Saccharomycotina, Saccharomycetes, Saccharomycetidae, Saccharomycetales, Debaryomycetaceae, Meyerozyma
Strain designation
B77
Type strain
No
Isolation source
Blood
Geographical isolation
Germany; North Rhine-Westphalia; Essen
Product format
Freeze-dried
General
Preceptrol
No
Characteristics
Morphology
After 3 days on YM medium at 25°C, colony is white to cream-colored, smooth, butyrous. Cells globose or ovoidal, single or in pairs, pseudohyphae not observed.
Comments
In vitro susceptibility (MIC): 0.06 mg/L itraconazole; 2 mg/L fluconazole; 0.125 mg/L amphotericin B
Handling information
Medium
ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 200: YM agar or YM broth
ATCC Medium 1245: YEPD
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature for (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium.
Incubate the inoculum/strain at the temperature and conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
In vitro susceptibility (MIC): 0.06 mg/L itraconazole; 2 mg/L fluconazole; 0.125 mg/L amphotericin B; the species is also known as Meyerozyma (= Pichia) guilliermondii.
Additional, updated information on this product may be available on the ATCC web site at www.atcc.org.
