Borrelia japonica Kawabata et al.
51557 ™
Product category
Bacteria
Strain designation
JCM 8951 [HO14]
Type strain
Yes
Isolation source
Tick (Ixodes ovatus)
Geographical isolation
Japan
Applications
Infectious disease research
Vector-borne disease research
Product format
Frozen
Storage conditions
-80°C or colder
General
Preceptrol
No
Handling information
Medium
ATCC Medium 1914: Revised BSK medium
Temperature
30°C
Atmosphere
Microaerophilic
Handling procedure
Thaw and aseptically transfer the entire contents of the vial to a tube of fresh #1914 broth (5 to 6 mL). Mix well.
Transfer one-tenth of the cell suspension to two or three additional tubes of fresh #1914 broth.
Incubate tubes at 30°C for 3-7 days.
Handling notes
Growth is usually established after 3 to 7 days. Acid formation during growth will change the medium from red to orange to yellow. Turbidity is not evident. Cells can be monitored under phase microscopy as long spiral rods with twitching motility.
Borrelia japonica is a fragile, sensitive organism that must have the appropriate medium for growth. Rabbit serum is essential for the growth of this organism. Fresh medium enhances growth. Medium older than one month should not be used. Additional transfers may be necessary to achieve good growth.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
History
Deposited as
Borrelia japonica Kawabata et al.
Depositors
JCM
Chain of custody
ATCC <-- JCM <-- T. Matsuzawa HO14
Type of isolate
Arthropod
Special collection
NCRR Contract
Cross references
GenBank BORRGDB Borrelia japonica 16S ribosomal RNA (16S rRNA) gene
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
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