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VR-1853 ™

 

Product category

Bacteria

Classification

Thiotrichales, Francisellaceae

Strain designation

Original

Isolation source

Malpighian tubules of ticks, Argas persicus (Oken) now Argas Persicargas arboreus, removed from buff-backed heron Bubulcus ibis.

Geographical isolation

United Arab Republic

Applications

Infectious disease research

Vector-borne disease research

Product format

Frozen

Storage conditions

-70°C or colder

General

Specific applications

Vector-borne pathogens research

Characteristics

Comments

Derived by adaptation of egg culture product ATCC® VR-331™ to Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™). ATCC® VR-331™ and ATCC® VR-1853™ have not been compared for sequence or infectivity in egg culture and tissue culture.

Handling information

Host

Aedes albopictus clone C6/36 (ATCC CRL-1660)

Effects

cell rounding; cell sloughing; CPE

Complete medium

EMEM (ATCC® 30-2003™) + 5% FBS (ATCC® 30-2020™)

Temperature

28°C

Incubation

6-8 days at 28°C in a humidified, air atmosphere incubator, until CPE is progressed through 80% of the monolayer.

Recommendation for infection

Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 28°C in a humidified, air atmosphere incubator. End adsorption by adding virus growth medium.

Handling notes

Derived by adaptation of egg culture product ATCC® VR-331™ to Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™). ATCC® VR-331™ and ATCC® VR-1853™ have not been compared for sequence or infectivity in egg culture and tissue culture.

Key abbreviations

°C, Degrees Celsius

CO2, Carbon dioxide

EMEM, Eagle's Minimum Essential Medium

FBS, Fetal bovine serum

MOI, Multiplicity of infection

Quality control specifications

Volume

1.0 mL

History

Deposited as

Wolbachia persica Suitor and Weiss

Depositors

E Weiss, Naval Medical Research Center

Type of isolate

Arthropod

Cross references

GenBank M21292 Wolbachia persica 16S ribosomal RNA.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

 

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

 

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

 

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.