Product category
Viruses
Product type
Animal virus
Strain designation
68-CV11
Genome sequenced strain
Yes
Isolation source
Derived from NIAID reagent V-127-001-021 (VR-117) by passage at ATCC.
Applications
Infectious disease research
Respiratory disease research
Product format
Frozen
Storage conditions
-70°C or colder
General
Specific applications
Respiratory research
Characteristics
Comments
Optimal conditions for grwoth include pH 8.6-7.3 in roller or rocker cultures incubated at approximately 33°C. All members of the rhinovirus group are ether resistant.
Mycoplasma contamination
Not detected
Handling information
Host
HeLa (ATCC CCL-2)
Alternate host(s): HD, HEK, MRC-5, MkK, WI-38
Effects
cell clustering; cell rounding; cell sloughing; CPE
Complete medium
EMEM (ATCC® 30-2003™) + 2% FBS (ATCC® 30-2020™)
Temperature
33°C
Atmosphere
95% Air, 5% CO2
Incubation
1 to 7 days at 33°C, rocker, in a humidified, 5% CO2 in air atmosphere.
Recommendation for infection
For best results plant cells 24 to 48 hours prior to infections, and infect at 70% - 80% confluence at a MOI of 0.1-1.
Handling notes
Derived by passage of NIAID reagent V-127-001-021 (VR-1117) at ATCC. Optimal conditions for growth include pH 6.8-7.3 in roller or rocker cultures incubated at approximately 33°C. All members of the rhinovirus group are ether resistant.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
HD, Human diploid cells
HEK, Human embryonic kidney cells
HeLa, Human cervical carcinoma cells
MkK, Monkey kidney
MRC-5, Human embryonic lung cells
MOI, Multiplicity of infection
NIAID, National Institute of Allergy and Infectious Diseases
WI-38, Human embryonic lung (diploid) cells
Quality control specifications
History
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