Chikungunya virus strain 181/25 is an attenuated strain propagated in Vero C1008 cells (ATCC CRL-1586). This animal virus was isolated in 1962 from a human in Thailand.
Product category
Viruses
Product type
Animal virus
Classification
Togaviridae, Alphavirus
Strain designation
181/25
Genome sequenced strain
Yes
Isolation source
Human
Geographical isolation
Thailand
Applications
Infectious disease research
Vector-borne disease research
Product format
Frozen
Storage conditions
-70°C or colder
Comments
Chikunguya virus, strain 181/25 is an attenuated derivative of strain AF15561, which was originally isolated from a human in Thailand in 1962. Attenuation of this strain is mediated by two amino acid substitutions in the E2 glycoprotein.
Host
VERO C1008 [Vero 76, clone E6, Vero E6] (ATCC CRL-1586)
Effects
cell rounding; cell sloughing; cell detachment
Complete medium
DMEM (ATCC 30-2002) + 2% FBS (ATCC 30-2020)
Temperature
37°C
Incubation
4-8 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer.
Recommendation for infection
Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes
Chikunguya virus, strain 181/25 is an attenuated derivative of strain AF15561, which was originally isolated from a human in Thailand in 1962. Attenuation of this strain is mediated by two amino acid substitutions in the E2 glycoprotein.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
DMEM, Dulbecco's Modified Eagle's Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
Volume
1.0 mL
Verification method
Whole-genome Sequencing
Depositors
C Schmaljohn
Type of isolate
Human
Year of origin
1962
Cross references
GenBank MW473668.1 Chikungunya virus strain 181/25, complete genome
