Yarrowia lipolytica (Wickerham et al.) van der Walt et von Arx
18942
Product category
Fungi
Product type
Yeast
Strain designation
NRRL YB-423 [CBS 6124, DBVPG 6053, IFO 1548, JCM 2320]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Corn processing plant
Geographical isolation
United States; Illinois
Applications
Industrial biotechnology
Bioremediation
Biofuel production
Food production research
Product format
Freeze-dried
Storage conditions
2°C to 8°C
Specific applications
produces lipase
produces lipases active at pH 9.0 at 60C
Potential biocontrol agent: vegetable and mineral oil waste
Potential biofuel production agent: biodiesel
Potential strain for oil or fat production
Non-conventional yeasts for bioconversion and precision fermentation
Preceptrol
No
Characteristics
Comments
Bisexual
Handling information
Medium
ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth
ATCC Medium 200: YM agar or YM broth
ATCC Medium 1245: YEPD
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
For freeze-dry (lyophilized) ampoules:
Open an ampoule according to enclosed instructions.
From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
Aseptically transfer the suspension back into the test tube of sterile distilled water.
Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
Incubate the inoculum at the propagation conditions recommended.
Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
