Klebsiella pneumoniae strain KP1074 [B21] is a whole-genome sequenced bacterium that was isolated from a human with leukemia/lymphoma in Tel-Hashomer, Israel. This strain produces Klebsiella pneumoniae carbapenemase (KPC-3). The depositor states that this strain can be used as a QC reference strain for testing new beta-lactam/beta-lactamase inhibitor combinations such as meropenem/vaborbactam. The bacterium is a CLSI control strain for antimicrobial susceptibility testing.
General
Specific applications
Antibiotic QC testing
Strain produces Klebsiella pneumoniae carbapenemase (KPC-3)
The depositor states that this strain can be used as a QC reference strain for testing new beta-lactam/beta-lactamase inhibitor combinations, such as meropenem/vaborbactam
This is a CLSI control strain for antimicrobial susceptibility testing.
Preceptrol
No
Characteristics
Genotype
blaKPC-3 positive
Susceptibility profile
Resistant
Amikacin
Fluoroquinolone
Tobramycin
Comments
Klebsiella pneumoniae Carbapenemase (KPC) positive
blaKPC-3 positive by PCR
Resistant to beta-lactams
Handling information
Medium
ATCC Medium 3: Nutrient agar or nutrient broth
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
Aseptically transfer the entire contents to a 5-6 mL tube of #3 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
Use several drops of the primary broth tube to inoculate a #3 plate and/or #3 agar slant.
Incubate at 37°C for 24 hours.
Handling notes
The depositor states that this strain can be used as a QC reference strain for testing new beta-lactam/beta-lactamase inhibitor combinations, such as meropenem/vaborbactam.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.