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平臺編號：bio-46869
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國際編號：ATCC PRA-98
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拉丁屬名： Trichomonas vaginalis Donne
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Trichomonas vaginalis Donne
Trichomonas vaginalis Donne (ATCC? PRA-98?)
Permits and Restrictions
View Permits
Strain DesignationsG3
Application
Sexually Transmitted Disease Research
Biosafety Level2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
IsolationClinical specimen - human, Beckenham United Kingdom, 1973
Product Formatfrozen
Storage ConditionsFrozen Cultures:
-70℃ for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures:
2-8℃
Live Cultures:
See Protocols section for handling information
Type Strainno
Genome Sequenced Strain
Yes
Comments
Genome sequencing strain
In vitro susceptibility to metronidazole
MediumATCC? Medium 2154: LYI Entamoeba medium
ATCC? Medium 361: Modified TYM basal medium (ATCC medium 358) with pH adjusted to 6.0 and 0.2-0.5 ml of heat-inactivated horse serum added per tube before use
Growth ConditionsTemperature: 35℃
Atmosphere: Anaerobic
Culture System: Axenic; pH 6
CryopreservationHarvest and Preservation
Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. The cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.
Adjust the concentration of cells to 2 x 106 to 2 x 107/mL in fresh medium.
While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium.
Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube.
Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium.
Invert several times to dissolve the DMSO.
Allow to warm to room temperature.
Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 to 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.
Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place the vials in a controlled rate freezing unit. From room temperature cool at -1℃/min to -40℃. If the freezing unit can compensate for the heat of fusion, maintain rate at -1℃/min through the heat of fusion. At -40℃ plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1℃ freezing apparatus. Place the apparatus at -80℃ for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1℃/min.)
The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130℃ are stabile indefinitely. Those stored at temperatures above -130℃ are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55℃.
To establish a culture from the frozen state place an ampule in a water bath set at 35℃. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 361 (completed with serum) or 13 mL ATCC Medium 2154 adjusted to pH 6.0.
Incubate the culture at 35℃ with the cap screwed on tightly (tube should be vertical for medium 361 or on a 15° horizontal slant for medium 2154).
