ATCC? Number:PRA-212?
Organism: Naegleria pringsheimi De Jonckheere
Designations: NB-1 [Naegleria gruberi NB-1]
Isolation: England, United Kingdom
Depositors: C Walsh
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 711: PYBTemperature: 34.0°C Max Temperature: 35.0°C Min Temperature: 25.0°C Duration: grown with Klebsiella pneumoniae (i.e., culture is bacterized) Protocol: his strain is distributed as a frozen preparation. See the general procedures for thawing a frozen vial. Once vial is thawed, aseptically transfer the entire contents to the center of an agar plate of ATCCmedium 711 and spread it evenly over the surface of the plate with a spread bar. Incubate the plate at 25C. Trophozoites (amoebae) should be evident within 2-3 days.
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