ATCC? Number:PRA-181?
Organism: Perkinsus olseni
Designations: Mie-5MG/F8
Isolation: marine invertebrate Mie, Japan Isolation date: May 29, 2002
Depositors: C Dungan
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 1886: Perkinsus broth mediumTemperature: 25.0°C Max Temperature: 28.0°C Min Temperature: 25.0°C Duration: axenic Protocol: Aseptically transfer 0.1 ml of the culture to 10.0 ml of fresh medium in a 25 cm2 tissue culture flask. Incubate at 25C. Transfer cultures every two weeks. Interval: 14 days
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: This species was isolated from mantle/gill tissue from the Manila Clam, Venerupis ( = Tapes, Ruditapes) philippinarum.
Cross References: Nucleotide (GenBank) : DQ516679LSU rRNANucleotide (GenBank) : DQ516693Actin geneNucleotide (GenBank) : DQ516706ITS rDNA
References: 92211: . Synonymy of Perkinsus olseni Lester & Davis, 1981 and P. atlanticus Azevedo, 1989 and an update on the phylogenetic position of the genus Perkinsus . Bull. Eur. Ass. Fish Pathol. 22: 258-265, 2002. 92479: Dungan C F, Reece K S. In vitro propagation of two Perkinsus spp. parasites from japanese manila clams Venerupis philippinarum, and description of Perkinsus honshuensis n. sp.. J. Eukaryot. Microbiol. in press: 2006..