ATCC? Number:PRA-103?
Organism: Acanthoeca spectabilis (Acanthoeca spectabilis)
Designations: Ellis
Isolation: April 18, 1996 from salt marsh in Swan Bay, Queenscliffe, Australia.
Depositors: B Leadbeater
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1525: Seawater 802 mediumTemperature: 20.0℃ Growth condition: monoxenic with Enterobacter aerogenes ATCC13048. Protocol: Thaw the frozen material in a 35.0℃ water bath and transfer the contents into a T25 flask containing 10 ml of growth medium. Flagellates should be observed within 4-6 days. Subculture on a weekly basis by scraping the bottom of the flask with a cell scraper and transferring one tenth of the culture into a new flask with medium.
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Comments: Phylogenetic analyses of SSU rRNA, LSU rRNA, tubA, and hsp-90 [16172870]
References: 16172838: Leadbeater BS, et al. Choanoflagellate lorica construction and assembly: the nudiform condition. II. Acanthoeca spectabilis Ellis. Protist 159: 495-505, 2008. PubMed: 18485816 16172839: Leadbeater BS. Developmental and ultrastructural observations on two stalked marine choanoflagellates, Acanthoecopsis spiculifera Norris and Acanthoeca spectabilis Ellis. Proc. R. Soc. Lond. B Biol. Sci. 204: 57-66, 1979. PubMed: 37513 16172870: Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774