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平臺編號：bio-47043
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國際編號：ATCC PRA-138
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拉丁屬名： Neospora caninum
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
犬新孢子蟲
ATCC? Number:PRA-138?
Organism: Neospora caninum Dubey et al.
Designations: NC-Bahia
Isolation: clinical specimen - animal (dog (Canis familiaris))Salvador Brazil Isolation date: October, 1999
Depositors: LF Gondim, MM McAllister
Biosafety Level:2
Shipped: frozen
Growth Conditions: Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0℃ Duration: The organism is propagated in Vero cells. Protocol:
- Scrape the Vero cells into the culture medium when holes develop in the monolayer (tachyzoite clusters are observed in the cells and 7 micrometer long banana-shaped tachyzoites are free in the medium).
- Transfer approximately 0.5 ml of the harvested medium (or approximately 500,000 tachyzoites) to another T25 flask containing a fresh monolayer of vero cells.
- Incubate at 37C.
Interval: approximately 1 week
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cross References: Nucleotide (GenBank) : AY259043Neospora caninum strain NC-Bahia internal transcribed spacer 1, complete sequenceNucleotide (GenBank) : AY259042Neospora caninum strain NC-Bahia internal transcribed spacer 1, complete sequence
References: 50692: Barber JS, et al. Characterization of the first European isolate of Neospora caninum (Dubey, Carpenter, Speer, Topper and Uggla). Parasitology 111: 563-568, 1995. PubMed: 8559588 91769: Gondim LF, et al. Isolation of Neospora caninum from the brain of a naturally infected dog, and production of encysted bradyzoites in gerbils. Vet. Parasitol. 101: 1-7, 2001. PubMed: 11587828 91770: Gondim LF, et al. Variation of the internal transcribed spacer 1 sequence within individual strains and among different strains of Neospora caninum. J. Parasitol. 90: 119-122, 2004. PubMed: 15040677
