ATCC? Number:PRA-109?
Organism: Brachiola algerae
Designations: Human Muscle Isolate
Isolation: clinical specimen - human (human muscle (deltoid) biopsy)Pennsylvania, Isolation date: January 7, 2003
Depositors: LM Weiss, CM Coyle
History: L M Weiss C M Coyle
Biosafety Level:2
Shipped: frozen
Growth Conditions: Temperature: 32.0°C Duration: RK13 cells, use Eagle's minimum essential medium (EMEM);L6E9 cells, use Dulbecco's modified Eagle's medium (DMEM) Protocol: place spores on fresh cell monolayer Interval: monthly
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cross References: Nucleotide (GenBank) : AY230191Brachiola algerae small subunit ribosomal RNA gene and intergenic spacer region, complete sequence; and large subunit ribosomal RNA gene, partial sequence
References: 90197: Coyle CM, et al. Fatal myositis due to the microsporidian Brachiola algerae, a mosquito pathogen. N. Engl. J. Med. 351: 42-47, 2004. PubMed: 15229306 90198: Lowman PM, et al. The effects of elevated temperatures and various time-temperature combinations on the development of Brachiola (Nosema) algerae N. Comb. in mammalian cell culture. J. Eukaryot. Microbiol. 47: 221-234, 2000. PubMed: 10847338