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平臺(tái)編號(hào)：bio-47059
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國際編號(hào)：ATCC PRA-105
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拉丁屬名： Acanthamoeba castellanii
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Acanthamoeba castellanii*
ATCC? Number:PRA-105?
Organism: Acanthamoeba castellanii (Douglas) Page
Designations: 1BU
Isolation: clinical specimen - human (cornea of patient with keratitis)Vienna Austria Isolation date: 1998
Depositors: J Walochnik
History: J Walochnik
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCCmedium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days. Interval: every month
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cross References: Nucleotide (GenBank) : AF260721Acanthamoeba castellanii strain 1BU 18S small subunit ribosomal RNA gene, partial sequence
Classification: KINGDOM: Protozoa
References: 90184: Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891 90185: Walochnik J, et al. Immunological inter-strain crossreactivity correlated to 18S rDNA sequence types in Acanthamoeba spp. Int. J. Parasitol. 31: 163-167, 2001. PubMed: 11239936 90186: Walochnik J, et al. Cytotoxic activities of alkylphosphocholines against clinical isolates of Acanthamoeba spp. Antimicrob. Agents Chemother. 46: 695-701, 2002. PubMed: 11850250
Organism: Acanthamoeba castellanii (Douglas) Page
Designations: 1BU
Isolation: clinical specimen - human (cornea of patient with keratitis)Vienna Austria Isolation date: 1998
Depositors: J Walochnik
History: J Walochnik
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 712: PYGTemperature: 25.0°C Duration: axenic Protocol: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium containing 12% (w/v) sucrose to the freeze-dried inner shell vial. Once the culture is completely rehydrated, aseptically add 1 ml of ATCCmedium 712 and distribute to a 16 X 125 mm plastic screw-capped test tube or a T-25 tissue culture flask containing 5.0 ml of the same medium. Incubate the test tube culture horizontally with the cap on tight. Trophozoites should be evident in 1-5 days. Interval: every month
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cross References: Nucleotide (GenBank) : AF260721Acanthamoeba castellanii strain 1BU 18S small subunit ribosomal RNA gene, partial sequence
Classification: KINGDOM: Protozoa
References: 90184: Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891 90185: Walochnik J, et al. Immunological inter-strain crossreactivity correlated to 18S rDNA sequence types in Acanthamoeba spp. Int. J. Parasitol. 31: 163-167, 2001. PubMed: 11239936 90186: Walochnik J, et al. Cytotoxic activities of alkylphosphocholines against clinical isolates of Acanthamoeba spp. Antimicrob. Agents Chemother. 46: 695-701, 2002. PubMed: 11850250
