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平臺編號：bio-47060
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國際編號：ATCC 75710
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拉丁屬名： Neospora caninum
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Neospora caninum
ATCC? Number:75710?
Organism: Neospora caninum Dubey et al.
Designations: BPA1
Isolation: brain and/or spinal cord of an aborted bovine fetus [32658]
Depositors: K Sverlow
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 722: Minimum essential medium (MEM)Temperature: 35.0°C Protocol: This strain is routinely shipped as a frozen stabilate. See the general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the material to a T-25 tissue culture flask containing a monolayer of monkey kidney cells (Vero cells, ATCCCCL-81). To subculture, scrape the monolayer with a cell scraper to detach adhering cells, agitate, and aseptically transfer 0.25 ml to 10 ml of a fresh monolayer of Vero cells. Outgas the flask for 10 seconds with 95% air, 5% CO2. Screw the cap on tightly for optimal results and incubate at 35C under 5% CO2.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: detection of Neospora infections in cattle and other animals [32658]
References: 32658: Conrad PA, et al. Bovine Neospora isolates. US Patent 5,707,617 dated Jan 13 1998
Organism: Neospora caninum Dubey et al.
Designations: BPA1
Isolation: brain and/or spinal cord of an aborted bovine fetus [32658]
Depositors: K Sverlow
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 722: Minimum essential medium (MEM)Temperature: 35.0°C Protocol: This strain is routinely shipped as a frozen stabilate. See the general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the material to a T-25 tissue culture flask containing a monolayer of monkey kidney cells (Vero cells, ATCCCCL-81). To subculture, scrape the monolayer with a cell scraper to detach adhering cells, agitate, and aseptically transfer 0.25 ml to 10 ml of a fresh monolayer of Vero cells. Outgas the flask for 10 seconds with 95% air, 5% CO2. Screw the cap on tightly for optimal results and incubate at 35C under 5% CO2.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: detection of Neospora infections in cattle and other animals [32658]
References: 32658: Conrad PA, et al. Bovine Neospora isolates. US Patent 5,707,617 dated Jan 13 1998
