ATCC? Number:50929?
Organism: Salpingoeca pyxidium Kent
Designations: BSA-03190006
Isolation: salt marsh sediment, Hog Island, eastern shore of Virginia, 1999 [178444]
Depositors: TA Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1525: Seawater 802 mediumTemperature: 25.0°C Duration: Xenic, grown with Klebsiella pneumoniae subsp. pneumoniae ATCC700831 and mixed bacteria Protocol: To subcultivate, scrape the bottom of the flask at or near peak density with a cell scraper, vigorously agitate the flask, and aseptically transfer a 0.25-ml aliquot to a T-25 flask containing 10 ml of ATCCmedium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae ATCC700831.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Phylogenetic analyses of SSU rRNA, LSU rRNA, tubA, and hsp-90 [16172870]
References: 178444: Thomas A Nerad, personal communication 16172870: Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774