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平臺編號：bio-47129
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國際編號：ATCC 50926
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拉丁屬名： Dermamoeba minor
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Dermamoeba minor
ATCC? Number:50926?
Organism: Dermamoeba minor (Pussard et al.) Page
Designations: GMU-1
Isolation: pond sediment, George Mason University, Manassas, VA, 2001 [178444]
Depositors: TA Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 919: Non-nutrient agarTemperature: 25.0°C Protocol: Both ATCC50926 and the food organism, Chlorella sp. ATCC30562, are shipped frozen. Upon their arrival, establish a culture of ATCC30562 as soon as possible. It is best to order ATCC30562 in advance of ordering ATCC50926 unless you have liquid nitrogen storage facilities. When ATCC30562 arrives, place it directly into a 35C-water bath and transfer its thawed contents to a fresh plate of ATCCmedium 5. Incubate the plate at 25C under a 14/10-hour light cycle under 50-100 microEinsteins/meter squared per second of irradiance. When there is a dense lawn of cells, suspend the cells using ATCCmedium 1323 or some other balanced salt solution. Transfer a 0.2-ml aliquot of a dense suspension of the alga to an agar plate of ATCCmedium 919 and spread it evenly over the surface with a spread bar. Thaw the frozen ampule of ATCC50926 in a 35C-water bath. Transfer the entire contents of the vial to the agar plate of ATCCmedium 919 with the lawn of Chlorella. Incubate the plate upright in the dark at 25C. Maintain cultures of the food organism on ATCCmedium 5 as described above.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
References: 178444: Thomas A Nerad, personal communication
Organism: Dermamoeba minor (Pussard et al.) Page
Designations: GMU-1
Isolation: pond sediment, George Mason University, Manassas, VA, 2001 [178444]
Depositors: TA Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 919: Non-nutrient agarTemperature: 25.0°C Protocol: Both ATCC50926 and the food organism, Chlorella sp. ATCC30562, are shipped frozen. Upon their arrival, establish a culture of ATCC30562 as soon as possible. It is best to order ATCC30562 in advance of ordering ATCC50926 unless you have liquid nitrogen storage facilities. When ATCC30562 arrives, place it directly into a 35C-water bath and transfer its thawed contents to a fresh plate of ATCCmedium 5. Incubate the plate at 25C under a 14/10-hour light cycle under 50-100 microEinsteins/meter squared per second of irradiance. When there is a dense lawn of cells, suspend the cells using ATCCmedium 1323 or some other balanced salt solution. Transfer a 0.2-ml aliquot of a dense suspension of the alga to an agar plate of ATCCmedium 919 and spread it evenly over the surface with a spread bar. Thaw the frozen ampule of ATCC50926 in a 35C-water bath. Transfer the entire contents of the vial to the agar plate of ATCCmedium 919 with the lawn of Chlorella. Incubate the plate upright in the dark at 25C. Maintain cultures of the food organism on ATCCmedium 5 as described above.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
References: 178444: Thomas A Nerad, personal communication
