ATCC® Number:50841™
Organism: Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux
Designations: PTG [PLK]
Isolation: high passage line, cloned from ME49 [112383]
Depositors: LD Sibley
History: ATCC<<--LD Sibley<<--J.C. Boothroyd <<-- L. Kasper
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 2222: 7049 7049Temperature: 35.0°C Duration: grown with human foreskin fibroblast cells, ATCCCRL-1634 Protocol: with serum reduced to 3%
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Geno Type: Type II, SAG1-2; SAG2-2; SAG3-2 [112383]
Comments: clonal lineage [50660] [50661] RFLP linkage map [50659] [50658] Genotype: Type II, SAG1-2; SAG2-2; SAG3-2 [112383]
Classification: KINGDOM: Protozoa
References: 50658: Howe DK, Sibley LD. Toxoplasma gondii: analysis of different laboratory stocks of the RH strain reveals genetic heterogeneity. Exp. Parasitol. 78: 242-245, 1994. PubMed: 7907030 50659: Sibley LD, et al. Generation of a restriction fragment length polymorphism linkage map for Toxoplasma gondii. Genetics 132: 1003-1015, 1992. PubMed: 1360931 50660: Howe DK, Sibley LD. Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease. J. Infect. Dis. 172: 1561-1566, 1995. PubMed: 7594717 50661: Sibley LD, Boothroyd JC. Virulent strains of Toxoplasma gondii comprise a single clonal lineage. Nature 359: 82-85, 1992. PubMed: 1355855 53516: Howe DK, et al. Acute virulence in mice is associated with markers on chromosome VIII in Toxoplasma gondii. Infect. Immun. 64: 5193-5198, 1996. PubMed: 8945565 64426: Howe DK, et al. Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis . J. Clin. Microbiol. 35: 1411-1414, 1997. PubMed: 9163454 64442: Mordue DG, et al. Acute toxoplasmosis leads to lethal overproduction of Th1 cytokines. J. Immunol. 167: 4574-4584, 2001. PubMed: 11591786 64444: Su C, et al. Identification of quantitative trait loci controlling acute virulence in Toxoplasma gondii. Proc. Natl. Acad. Sci. USA 99: 10753-10758, 2002. PubMed: 12149482 64452: Mordue DG, et al. Invasion by Toxoplasma gondii establishes a moving junction that selectively excludes host cell plasma membrane proteins on the basis of their membrane anchoring. J. Exp. Med. 190: 1783-1792, 1999. PubMed: 10601353 69266: Barragan A, Sibley LD. Transepithelial migration of Toxoplasma gondii is linked to parasite motility and virulence. J. Exp. Med. 195: 1625-1633, 2002. PubMed: 12070289 70374: Asai T, et al. Biochemical and molecular characterization of nucleoside triphosphate hydrolase isozymes from the parasitic protozoan Toxoplasma gondii. J. Biol. Chem. 270: 11391-11397, 1995. PubMed: 7744775 70399: Sibley LD, et al. Genetic approaches to studying virulence and pathogenesis in Toxoplasma gondii. Philos. Trans. R. Soc. Lond. B Biol. Sci. 357: 81-88, 2002. PubMed: 11839185 112383: L D Sibley, personal communication
ATCC50841
