ATCC? Number:50839?
Organism: Toxoplasma gondii (Nicolle and Manceaux) Nicolle and Manceaux
Designations: 2F [RH-2F]
Isolation: cloned from RH-88, ATCC50838 [112383]
Depositors: LD Sibley
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 2222: 7049 7049Temperature: 35.0°C Growth condition: Grown in human foreskin fibroblast cell line ATCCCRL-1634 with serum reduced to 3%
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Geno Type: Type I, SAG1-1; SAG2-1; SAG3-1 [112383]
Comments: clonal lineage Genotype: Type I, SAG1-1; SAG2-1; SAG3-1 [112383] Contains the 脽-galactosidase gene under the control of the GRA1 promoter [48383] [112383]
Classification: KINGDOM: Protozoa
References: 48383: Carruthers VB, et al. Toxoplasma gondii uses sulfated proteoglycans for substrate and host cell attachment. Infect. Immun. 68: 4005-4011, 2000. PubMed: 10858215 62907: . Toxoplasma gondii microneme secretion involves intracellular Ca(2+) release from inositol 1,4,5-triphosphate (IP(3))/ryanodine-sensitive stores. J. Biol. Chem. 277: 25870-25876, 2002. PubMed: 12011085 64077: . Identification of small-molecule inhibitors of nucleoside triphosphate hydrolase in Toxoplasma gondii. Antimicrob. Agents Chemother. 46: 2393-2399, 2002. PubMed: 12121910 64445: Carruthers VB, et al. The Toxoplasma adhesive protein MIC2 is proteolytically processed at multiple sites by two parasite-derived proteases. J. Biol. Chem. 275: 14346-14353, 2000. PubMed: 10799515 69266: Barragan A, Sibley LD. Transepithelial migration of Toxoplasma gondii is linked to parasite motility and virulence. J. Exp. Med. 195: 1625-1633, 2002. PubMed: 12070289 70398: Wetzel DM, et al. Actin filament polymerization regulates gliding motility by apicomplexan parasites. Mol. Biol. Cell 14: 396-406, 2003. PubMed: 12589042 112383: L D Sibley, personal communication