ATCC? Number:50594?
Organism: Colpodella sp.
Designations: GSP 10
Isolation: brown woodland soil, Gambrill State Park, Frederick, MD, 1993
Depositors: TK Sawyer
History: ATCC<<--TK Sawyer<<--T.A. Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumTemperature: 25.0°C Protocol: ATCCNO: 50594 SPEC: This strain is shipped as a diprotist culture with Bodo caudatus supplied as a food source. Mixed unidentified bacteria are also present as a food source for the prey organism. Upon receipt of the dried vial, open according to instructions enclosed and rehydrate by adding 0.5 ml of ATCCmedium 802. Aseptically remove the shredded filter paper pellet with a pair of sterile forceps and transfer to 10 ml of ATCCmedium 802 in a T-25 flask. Using a Pasteur pipette, aseptically transfer the remainder of the liquid from the vial to the T-25 flask. Agitate the flask to break apart the filter paper pellet, then incubate the culture at 25C with the cap screwed on tightly. On the following day, vigorously agitate the culture and aseptically transfer a 0.5-ml aliquot to 10 ml of ATCCmedium 802. The subculture will have almost completely encysted within 3-4 days. Cultures should be routinely subcultured every 14-21 days.
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Classification: KINGDOM: Protozoa