ATCC? Number:50604?
Organism: Encephalitozoon hellem Didier et al.
Isolation: urine from human, New York, 1992
Depositors: GS Visvesvara
History: ATCC<<--GS Visvesvara<<--CDC:V257
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 722: Minimum essential medium (MEM)Temperature: 35.0°C Duration: 5% CO2 Protocol: ATCCNO: 50451 SPEC: Thaw ampule in a 35C water bath and aseptically transfer contents to a T-25 tissue culture flask containing a monolayer of lung fibroblasts (ATCCCCL-75). Twice per week, remove the culture fluid and refeed the culture with fresh medium. Centrifuge the culture fluid at 1300 x g for 10 minutes, discard the supernatant, and transfer the resuspended cell pellet to a fresh monolayer of lung fibroblasts.
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Comments: Environmental resistance of spores [43281] flow cytometric analysis [38799] Control strain for enterocytozoan identification [43276] [43275]
References: 38799: Moss DM, et al. Flow cytometric analysis of microsporidia belonging to the genus Encephalitozoon. J. Clin. Microbiol. 37: 371-375, 1999. PubMed: 9889221 43275: del Aguila C, et al. Identification of Enterocytozoon bieneusi spores in respiratory samples from an AIDS patient with a 2-year history of intestinal microsporidiosis. J. Clin. Microbiol. 35: 1862-1866, 1997. PubMed: 9196210 43276: Croppo GP, et al. Identification of the microsporidian Encephalitozoon hellem using immunoglobulin G monoclonal antibodies. Arch. Pathol. Lab. Med. 122: 182-186, 1998. PubMed: 9499364 43281: Kucerova-Pospisilova Z, et al. Environmental resistance of Encephalitozoon spores. J. Eukaryot. Microbiol. 46: 11S-13S, 1999. PubMed: 10519227 70346: Xiao L, et al. Genotyping Encephalitozoon hellem isolates by analysis of the polar tube protein gene. J. Clin. Microbiol. 39: 2191-2196, 2001. PubMed: 11376056 70360: Sobottka I, et al. Acute and long-term humoral immunity following active immunization of rabbits with inactivated spores of various Encephalitozoon species. Parasitol. Res. 87: 1-6, 2001. PubMed: 11199842