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平臺編號：bio-47409
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國際編號：ATCC 50602
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拉丁屬名： Encephalitozoon cuniculi
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Encephalitozoon cuniculi
ATCC? Number:50602?
Organism: Encephalitozoon cuniculi Levaditi et al.
Designations: CDC:V282
Isolation: urine from AIDS patient, Colorado, 1993
Depositors: GS Visvesvara
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 722: Minimum essential medium (MEM)Temperature: 35.0°C Duration: 5% CO2 Protocol: ATCCNO: 50451 SPEC: Thaw ampule in a 35C water bath and aseptically transfer contents to a T-25 tissue culture flask containing a monolayer of lung fibroblasts (ATCCCCL-75). Twice per week, remove the culture fluid and refeed the culture with fresh medium. Centrifuge the culture fluid at 1300 x g for 10 minutes, discard the supernatant, and transfer the resuspended cell pellet to a fresh monolayer of lung fibroblasts.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Sporogony [43279] Environmental resistance of spores [43281] flow cytometric analysis [38799] detection of drug-induced effects in infected green monkey kidney cells [47325] Western blot and immunofluorescence analysis [38797] case report [21637] ITS RFLP analysis [18383] Control strain for enterocytozoan identification [43278] [43277]
References: 18383: Didier ES, et al. A microsporidian isolated from an AIDS patient corresponds to Encephalitozoon cuniculi III, originally isolated from domestic dogs. J. Clin. Microbiol. 34: 2835-2837, 1996. PubMed: 8897194 21637: De Groote MA, et al. Polymerase chain reaction and culture confirmation of disseminated Encephalitozoon cuniculi in a patient with AIDS: Successful therapy with albendazole. J. Infect. Dis. 171: 1375-1378, 1995. PubMed: 7751721 38797: Croppo GP, et al. Western blot and immunofluorescence analysis of a human isolate of Encephalitozoon cuniculi established in culture from the urine of a patient with AIDS. J. Parasitol. 83: 66-69, 1997. PubMed: 9057698 38799: Moss DM, et al. Flow cytometric analysis of microsporidia belonging to the genus Encephalitozoon. J. Clin. Microbiol. 37: 371-375, 1999. PubMed: 9889221 39769: Moura H, et al. Detection by an immunofluorescence test of Encephalitozoon intestinalis spores in routinely formalin-fixed stool samples stored at room temperature. J. Clin. Microbiol. 37: 2317-2322, 1999. PubMed: 10364604 43277: del Aguila C, et al. Ultrastructure, immunofluorescence, western blot, and PCR analysis of eight isolates of Encephalitozoon (Septata) intestinalis established in culture from sputum and urine samples and duodenal aspirates of five patients with AIDS. J. Clin. Microbiol. 36: 1201-1208, 1998. PubMed: 9574677 43278: Bornay-Llinares FJ, et al. Immunologic, microscopic, and molecular evidence of Encephalitozoon intestinalis (Septata intestinalis) infection in mammals other than humans. J. Infect. Dis. 178: 820-826, 1998. PubMed: 9728552 43279: Visvesvara GS, et al. Encephalitozoon cuniculi: light and electron microscopic evidence for di-, tetra, and octosporous sporogony and a note on the molecular phylogeny of Encephalitozoonidae. J. Eukaryot. Microbiol. 46: 110-115, 1999. PubMed: 10361732 43281: Kucerova-Pospisilova Z, et al. Environmental resistance of Encephalitozoon spores. J. Eukaryot. Microbiol. 46: 11S-13S, 1999. PubMed: 10519227 47325: Leitch GJ, et al. Use of fluorescent probe to assess the activities of candidate agents against intracellular forms of Encephalitozoon microsporidia. Antimicrob. Agents Chemother. 41: 337-344, 1997. PubMed: 9021189 70347: del Aguila C, et al. In vitro culture, ultrastructure, antigenic, and molecular characterization of Encephalitozoon cuniculi isolated from urine and sputum samples from a Spanish patient with AIDS. J. Clin. Microbiol. 39: 1105-1108, 2001. PubMed: 11230434 70360: Sobottka I, et al. Acute and long-term humoral immunity following active immunization of rabbits with inactivated spores of various Encephalitozoon species. Parasitol. Res. 87: 1-6, 2001. PubMed: 11199842 70446: Xiao L, et al. Genotyping Encephalitozoon cuniculi by multilocus analyses of genes with repetitive sequences. J. Clin. Microbiol. 39: 2248-2253, 2001. PubMed: 11376065
Organism: Encephalitozoon cuniculi Levaditi et al.
Designations: CDC:V282
Isolation: urine from AIDS patient, Colorado, 1993
Depositors: GS Visvesvara
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 722: Minimum essential medium (MEM)Temperature: 35.0°C Duration: 5% CO2 Protocol: ATCCNO: 50451 SPEC: Thaw ampule in a 35C water bath and aseptically transfer contents to a T-25 tissue culture flask containing a monolayer of lung fibroblasts (ATCCCCL-75). Twice per week, remove the culture fluid and refeed the culture with fresh medium. Centrifuge the culture fluid at 1300 x g for 10 minutes, discard the supernatant, and transfer the resuspended cell pellet to a fresh monolayer of lung fibroblasts.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Sporogony [43279] Environmental resistance of spores [43281] flow cytometric analysis [38799] detection of drug-induced effects in infected green monkey kidney cells [47325] Western blot and immunofluorescence analysis [38797] case report [21637] ITS RFLP analysis [18383] Control strain for enterocytozoan identification [43278] [43277]
References: 18383: Didier ES, et al. A microsporidian isolated from an AIDS patient corresponds to Encephalitozoon cuniculi III, originally isolated from domestic dogs. J. Clin. Microbiol. 34: 2835-2837, 1996. PubMed: 8897194 21637: De Groote MA, et al. Polymerase chain reaction and culture confirmation of disseminated Encephalitozoon cuniculi in a patient with AIDS: Successful therapy with albendazole. J. Infect. Dis. 171: 1375-1378, 1995. PubMed: 7751721 38797: Croppo GP, et al. Western blot and immunofluorescence analysis of a human isolate of Encephalitozoon cuniculi established in culture from the urine of a patient with AIDS. J. Parasitol. 83: 66-69, 1997. PubMed: 9057698 38799: Moss DM, et al. Flow cytometric analysis of microsporidia belonging to the genus Encephalitozoon. J. Clin. Microbiol. 37: 371-375, 1999. PubMed: 9889221 39769: Moura H, et al. Detection by an immunofluorescence test of Encephalitozoon intestinalis spores in routinely formalin-fixed stool samples stored at room temperature. J. Clin. Microbiol. 37: 2317-2322, 1999. PubMed: 10364604 43277: del Aguila C, et al. Ultrastructure, immunofluorescence, western blot, and PCR analysis of eight isolates of Encephalitozoon (Septata) intestinalis established in culture from sputum and urine samples and duodenal aspirates of five patients with AIDS. J. Clin. Microbiol. 36: 1201-1208, 1998. PubMed: 9574677 43278: Bornay-Llinares FJ, et al. Immunologic, microscopic, and molecular evidence of Encephalitozoon intestinalis (Septata intestinalis) infection in mammals other than humans. J. Infect. Dis. 178: 820-826, 1998. PubMed: 9728552 43279: Visvesvara GS, et al. Encephalitozoon cuniculi: light and electron microscopic evidence for di-, tetra, and octosporous sporogony and a note on the molecular phylogeny of Encephalitozoonidae. J. Eukaryot. Microbiol. 46: 110-115, 1999. PubMed: 10361732 43281: Kucerova-Pospisilova Z, et al. Environmental resistance of Encephalitozoon spores. J. Eukaryot. Microbiol. 46: 11S-13S, 1999. PubMed: 10519227 47325: Leitch GJ, et al. Use of fluorescent probe to assess the activities of candidate agents against intracellular forms of Encephalitozoon microsporidia. Antimicrob. Agents Chemother. 41: 337-344, 1997. PubMed: 9021189 70347: del Aguila C, et al. In vitro culture, ultrastructure, antigenic, and molecular characterization of Encephalitozoon cuniculi isolated from urine and sputum samples from a Spanish patient with AIDS. J. Clin. Microbiol. 39: 1105-1108, 2001. PubMed: 11230434 70360: Sobottka I, et al. Acute and long-term humoral immunity following active immunization of rabbits with inactivated spores of various Encephalitozoon species. Parasitol. Res. 87: 1-6, 2001. PubMed: 11199842 70446: Xiao L, et al. Genotyping Encephalitozoon cuniculi by multilocus analyses of genes with repetitive sequences. J. Clin. Microbiol. 39: 2248-2253, 2001. PubMed: 11376065
