-
平臺編號：bio-47424
-
國際編號：ATCC 50570
-
拉丁屬名： Hexamita sp.
-
規(guī)格：frozen
-
用途：ATCC原裝進(jìn)口
-
服務(wù)費(fèi)用：
加載中…… - 訂購
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Hexamita sp.
ATCC? Number:50570?
Organism: Hexamita sp.
Designations: HYMA-1
Isolation: sediment from freshwater pond, Hyannis, MA, 1995
Depositors: TA Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1773: Hexamita mediumTemperature: 25.0°C Duration: anaerobic Protocol: ATCCNO: 50570 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule, aseptically transfer the material to a 16 x 125 mm screw-capped test tube containing 12 ml of bacterized ATCCmedium 1773, screw the cap on tightly, and incubate on a 15 degree slant at 25C. Trophozoites should be active on the following day. Within 7-10 days most of the cells will encyst. The cysts remain viable for at least one month. However, to ensure viability, subculture every 14-21 days. To subculture, rub the surface of the tube with a sterile cotton swab, gently invert 10 times, aseptically transfer a 0.25-ml aliquot to a fresh tube of bacterized ATCCmedium 1773, and incubate as above. This strain is cultivated with a non-pathogenic strain of Enterobacter aerogenes ATCC-13048 as a food source. Other unidentified bacteria are also present. Note: ATCCmedium 1773 is bacterized by inoculating with a partial bacteriological loopful of a non-pathogenic strain of K. pneumoniae subsp. pneumoniae ATCC-27889 (other strains of Klebsiella will probably work equally well) from an ATCCmedium 3 slant approximately 24 hours before using. There is currently no known commercial source of rice starch that is suitable for inclusion in ATCCmedium 1773. However, purified rice starch of a suitable nature can be prepared (Diamond, L.S. Lumen dwelling protozoa: Entamoeba, trichomonads, and Giardia. In: Jensen, J.B. ed., In vitro cultivation of protozoan parasites. Boca Raton, FL: CRC Press; 1983:p. 75).
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Organism: Hexamita sp.
Designations: HYMA-1
Isolation: sediment from freshwater pond, Hyannis, MA, 1995
Depositors: TA Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1773: Hexamita mediumTemperature: 25.0°C Duration: anaerobic Protocol: ATCCNO: 50570 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule, aseptically transfer the material to a 16 x 125 mm screw-capped test tube containing 12 ml of bacterized ATCCmedium 1773, screw the cap on tightly, and incubate on a 15 degree slant at 25C. Trophozoites should be active on the following day. Within 7-10 days most of the cells will encyst. The cysts remain viable for at least one month. However, to ensure viability, subculture every 14-21 days. To subculture, rub the surface of the tube with a sterile cotton swab, gently invert 10 times, aseptically transfer a 0.25-ml aliquot to a fresh tube of bacterized ATCCmedium 1773, and incubate as above. This strain is cultivated with a non-pathogenic strain of Enterobacter aerogenes ATCC-13048 as a food source. Other unidentified bacteria are also present. Note: ATCCmedium 1773 is bacterized by inoculating with a partial bacteriological loopful of a non-pathogenic strain of K. pneumoniae subsp. pneumoniae ATCC-27889 (other strains of Klebsiella will probably work equally well) from an ATCCmedium 3 slant approximately 24 hours before using. There is currently no known commercial source of rice starch that is suitable for inclusion in ATCCmedium 1773. However, purified rice starch of a suitable nature can be prepared (Diamond, L.S. Lumen dwelling protozoa: Entamoeba, trichomonads, and Giardia. In: Jensen, J.B. ed., In vitro cultivation of protozoan parasites. Boca Raton, FL: CRC Press; 1983:p. 75).
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
