ATCC? Number:50582?
Organism: Giardia intestinalis (Lambl) Alexeieff
Designations: WB clone 1267
Depositors: TE Nash
Biosafety Level:2
Shipped: frozen
Growth Conditions: ATCCmedium 2155: LYI Giardia Medium (filtered)Temperature: 35.0°C Duration: axenic; anaerobic
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Serum Cohn Fraction IV-1 supports growth [21655] Triosephosphate isomerase gene [21656] Metabolism of cysteine [21660] Isoprenylation of proteins [21661] Induction of Golgi [21663] Lipid requirements [21664] Induction of differentiation by cholesterol starvation [21669] Expression of cyst wall protein gene [21705]
Classification: KINGDOM: Archeozoa
References: 21655: Lujan HD, et al. Serum Cohn fraction IV-1 supports the growth of Giardia lamblia in vitro. Infect. Immun. 62: 4664-4666, 1994. PubMed: 7927737 21656: Mowatt MR, et al. Complementation of an Escherchia coli glycolysis mutant by Giardia lamblia triosephosphate isomerase. Exp. Parasitol. 78: 85-92, 1994. PubMed: 8299763 21660: Lujan HD, Nash TE. The uptake and metabolism of cysteine by Giardia lamblia trophozoites. J. Eukaryot. Microbiol. 41: 169-175, 1994. PubMed: 8167619 21661: Lujan HD, et al. Isoprenylation of proteins in the protozoan Giardia lamblia. Mol. Biochem. Parasitol. 72: 121-127, 1995. PubMed: 8538683 21663: Lujan HD, et al. Developmental induction of Golgi structure and function in the primitive eukaryote Giardia lamblia. J. Biol. Chem. 270: 4612-4618, 1995. PubMed: 7876232 21664: Lujan HD, et al. Lipid requirements and lipid uptake by Giardia lamblia trophozoites in culture. J. Eukaryot. Microbiol. 43: 237-242, 1996. PubMed: 8640194 21669: Lujan HD, et al. Cholesterol starvation induces differentiation of the intestinal parasite Giardia lamblia. Proc. Natl. Acad. Sci. USA 93: 7628-7633, 1996. PubMed: 8755526 21705: Mowatt MR, et al. Developmentally regulated expression of a Giardia lamblia cyst wall protein gene. Mol. Microbiol. 15: 955-963, 1995. PubMed: 7596296 33583: Lujan HD, et al. Increased expression of the molecular chaperone BiP/GRP78 during the differentiation of a primitive eukaryote. Biol. Cell. 86: 11-18, 1996. PubMed: 8688827 70205: Nash T, Rice WG. Efficacies of zinc-finger-active drugs against Giardia lamblia. Antimicrob. Agents Chemother. 42: 1488-1492, 1998. PubMed: 9624499 70336: Elmendorf HG, et al. The abundance of sterile transcripts in Giardia lamblia. Nucleic Acids Res. 29: 4674-4683, 2001. PubMed: 11713317 70342: Touz MC, et al. Identification and characterization of a novel secretory granule calcium-binding protein from the early branching eukaryote Giardia lamblia. J. Biol. Chem. 277: 50557-50563, 2002. PubMed: 12354777 70343: Touz MC, et al. The activity of a developmentally regulated cysteine proteinase is required for cyst wall formation in the primitive eukaryote Giardia lamblia. J. Biol. Chem. 277: 8474-8481, 2002. PubMed: 11773053 70388: Lujan HD, et al. Identification of a novel Giardia lamblia cyst wall protein with leucine-rich repeats. Implications for secretory granule formation and protein assembly into the cyst wall. J. Biol. Chem. 270: 29307-29313, 1995. PubMed: 7493963 70389: Murtagh JJ Jr., et al. Guanine nucleotide-binding proteins in the intestinal parasite Giardia lamblia. Isolation of a gene encoding an approximately 20-kDa ADP-ribosylation factor. J. Biol. Chem. 267: 9654-9662, 1992. PubMed: 1577802