ATCC? Number:50535?
Organism: Nerada mexicana Cavalier-Smith and Chao
Isolation: dry soil, Mexico City, Mexico [100134]
Depositors: J Martinez-Cruz
History: ATCC<<--J Martinez-Cruz<<--T.A. Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1967: Soil freshwater cereal grass mediumTemperature: 25.0°C Protocol: This strain is shipped as a frozen stabilate on dry ice. Please read the instructions that accompany the shipment before handling the material. Thaw the vial as instructed and transfer its entire contents to 10 ml of ATCCmedium 1967 in a T-25 tissue culture flask. The bacterial flora present in the shipped culture will support growth, but growth may be improved by using medium that has been bacterized with Enterobacter aerogenes ATCC13048 approximately 24 hours before inoculation with the protist. (Other species of bacteria may work equally well, but this one has been empirically assessed.) Subculture every 14-21 days. To subculture, aseptically scrape the bottom of the flask, agitate the flask to distribute the cells evenly, and aseptically transfer 0.25 ml to 10 ml of bacterized medium in a T-25 flask. Screw the cap on tightly and incubate the flask at 25C.
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Type Strain: yes
Comments: Originally deposited as Pinaciophora sp. Name changed to Nerada mexicana as described in Cavalier-Smith and Chao (J Mol Evol 2006 62:388-420).
References: 100134: Jovita Martinez-Cruz, personal communication 16172750: Cavalier-Smith, T and EE Chao. Phylogeny and megasystematics of phagotrophic heterokonts (kingdom Chromista). J Mol Evol 62:388-420, 2006. PubMed: 16557340