ATCC? Number:50522?
Organism: Dimorpha sp.
Designations: CA-1
Isolation: hot spring (rich organic mud from stream, Palm Canyon, CA)California, United States Isolation date: 1994
Depositors: TA Nerad
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumMax Temperature: 45.0°C Min Temperature: 25.0°C Protocol: ATCCNO: 50522 SPEC: Place thawed contents of ampule into T-25 flask containing 10 ml of ATCCmedium 802 bacterized with Enterobacter aerogenes ATCC-13048. Subculture every 4-8 weeks (when maintained at 25C; cysts are stable longer at lower temperatures) as follows: Rub the surface of the agar with a sterile swab, agitate vigorously and aseptically transfer a 0.1 ml aliquot to a fresh flask of bacterized medium.
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Comments: Only heliomonad known to grow at 45C Monoprotist culture, cultivated with mixed bacteria and a non-pathogenic strain of Klebsiella pneumoniae subsp. pneumoniae ATCC27889 added as a food source.
Classification: KINGDOM: Protozoa