ATCC? Number:50517?
Organism: Ciliophrys infusionum Cienkowski
Isolation: seawater, Atlantic Ocean, Southampton, England, 1992
Depositors: S Tong
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1525: Seawater 802 mediumMax Temperature: 25.0°C Min Temperature: 10.0°C Protocol: ATCCNO: 50517 SPEC: Monoprotist culture, cultivated with mixed bacteria. Place thawed contents of ampule into T-25 flask containing 10 ml of ATCCmedium 802 bacterized with non-pathogenic Klebsiella pneumoniae subsp. pneumoniae ATCC-27889 as a food source. Subculture every 4-8 weeks (when maintained at 25C; cysts are stable longer at lower temperatures) as follows: Rub the surface of the agar with a sterile swab, agitate vigorously and aseptically transfer a 0.1 ml aliquot to a fresh flask of bacterized medium.
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Classification: KINGDOM: Chromista